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奥林巴斯显微镜荧光数据表

2014-08-12  发布者:admin 

  作为一个指南,激光共聚焦显微镜和广角荧光显微镜荧光团,下面列出了很多常用的荧光染料的表,与各自的峰值吸收和发射波长的激光光源和建议。 虽然作者承担的数据发表在各种可靠准确的报告责任,有几个注意事项必须考虑。 荧光染料对环境敏感的和不同的结果将与不同的溶剂和应用得到的。 在文献回顾,一个经常会发现相同的荧光色素提供不同的数据。 还有,在许多情况下,一个荧光染料的几个子品种。

该手册是荧光探针和化学品的研究 ,由Molecular Probes, Inc.发表 ,在俄勒冈州尤金市,是关于荧光染料信息的丰富来源。此外 ,请参照 Omega光学 色度技术

荧光染料的吸收峰值和发射波长
荧光染料 吸收波长 发射波长 Suggested
Laser
Acid Fuchsin
540  
630  
Green He-Ne
ACMA (Aminochloromethoxyacridine)
430  
474  
Blue Diode
Acridine Orange
(Bound to DNA)
502  
526  
Argon-Ion
Acridine Red
455-600      
560-680      
Argon-Ion
Acridine Yellow
470  
550  
Argon-Ion
Acriflavin
436  
520  
Blue Diode
AFA (Acriflavin Feulgen SITSA)
355-425      
460  
Blue Diode
Alexa Fluor 350
346  
442  
Helium-Cadmium
Alexa Fluor 405
401  
421  
Blue Diode
Alexa Fluor 430
433  
541  
Blue Diode
Alexa Fluor 488
495  
519  
Argon-Ion
Alexa Fluor 500
503  
525  
Argon-Ion
Alexa Fluor 514
518  
540  
Argon-Ion
Alexa Fluor 532
532  
553  
Krypton-Argon
Alexa Fluor 546
556  
573  
Green He-Ne
Alexa Fluor 555
555  
565  
Krypton-Argon
Alexa Fluor 568
578  
603  
Krypton-Argon
Alexa Fluor 594
590  
617  
Orange He-Ne
Alexa Fluor 610
612  
628  
Orange He-Ne
Alexa Fluor 633
632  
647  
Red He-Ne
Alexa Fluor 647
650  
665  
Krypton-Argon
Alexa Fluor 660
663  
690  
Red Diode
Alexa Fluor 680
679  
702  
Red Diode
Alizarin Complexon
530-560      
580  
Green He-Ne
Alizarin Red
530-560      
580  
Green He-Ne
AMCA
350  
445  
Helium-Cadmium
Aminoactinomycin D
555  
655  
Krypton-Argon
6-Aminoquinoline
355  
550  
Helium-Cadmium
Aniline Blue
370  
509  
Argon-Ion
ANS (Anilinonaphthalene Sulfonic Acid)
385  
485  
Blue Diode
Anthroyl Stearate
361-381      
446  
Argon-Ion
APC (Allophycocyanin)
650  
661  
Krypton-Argon
Astrazon Brilliant Red 4G
500  
585  
Argon-Ion
Astrazon Orange R
470  
540  
Argon-Ion
Astrazon Red 6B
520  
595  
Argon-Ion
Astrazon Yellow 7 GLL
450  
480  
Argon-Ion
Atabrine
436  
490  
Blue Diode
ATTO-TAG CBQ
465  
560  
Argon-Ion
ATTO-TAG FQ
486  
591  
Argon-Ion
Auramine O
460  
550  
Argon-Ion
Aurophosphine
450-490      
515  
Argon-Ion
Aurophosphine G
450  
580  
Argon-Ion
BAO 9-(Bisaminophenyloxadiazole)
365  
395  
Argon-Ion
BCECF
505  
530  
Argon-Ion
Berberine Sulphate
430  
550  
Blue Diode
Bisbenzamide
360  
600-610      
Helium-Cadmium
BOBO-1, BO-PRO-1
462  
481  
Argon-Ion
BOBO-3, BO-PRO-3
570  
604  
Krypton-Argon
Blancophor FFG Solution
390  
470  
Blue Diode
Blancophor SV
370  
435  
Argon-Ion
BODIPY FL
503  
512  
Argon-Ion
BODIPY TMR
542  
574  
Green He-Ne
BODIPY TR
589  
617  
Yellow He-Ne
BODIPY 492/515
490  
515  
Argon-Ion
BODIPY 493/503
500  
506  
Argon-Ion
BODIPY 500/510
509  
515  
Argon-Ion
BODIPY 505/515
502  
510  
Argon-Ion
BODIPY 530/550
533  
550  
Krypton-Argon
BODIPY 558/568
558  
568  
Krypton-Argon
BODIPY 564/570
564  
570  
Krypton-Argon
BODIPY 581/591
582  
590  
Yellow He-Ne
BODIPY 630/650
625  
640  
Red He-Ne
BODIPY 650/665
647  
665  
Red He-Ne
BODIPY 665/676
665  
676  
Red Diode
Brilliant Sulphoflavin FF
430  
520  
Blue Diode
BTC (Benzothiazole Calcium Indicator)
401, 464      
529  
Blue Diode
Calcein
494  
517  
Argon-Ion
Calcein Blue
370  
435  
Argon-Ion
Calcium Crimson
590  
611  
Yellow He-Ne
Calcium Green
505  
532  
Argon-Ion
Calcium Orange
549  
576  
Green He-Ne
Calcofluor (RW Solution)
370  
440  
Argon-Ion
Calcofluor White
440  
500-520      
Blue Diode
Calcofluor White (ABT Solution)
380  
475  
Argon-Ion
Calcofluor White (Standard Solution)
365  
435  
Argon-Ion
Carbocyanine LDL
520  
560  
Argon-Ion
5-(and 6-)-Carboxy SNARF-1 Indicator
548 (Low pH)  
576 (High pH)  
587 (Low pH)  
635 (High pH)  
Krypton-Argon
5-Carboxynaphthofluorescein
598  
668  
Yellow He-Ne
6-Carboxyrhodamine 6G
525  
555  
Argon-Ion
Cascade Blue
400  
425  
Blue Diode
Cascade Yellow
402  
545  
Blue Diode
Catecholamine
410  
470  
Blue Diode
CCF2 (GeneBLAzer)
402  
520  
Blue Diode
CellTracker Blue
353  
466  
Helium-Cadmium
CellTracker Green BODIPY
522  
529  
Argon-Ion
CFDA (Carboxyfluorescein Diacetate)
495  
520  
Argon-Ion
Chinacrine
450-490      
515  
Argon-Ion
Chromomycin A3
450  
570  
Argon-Ion
CL-NERF
504 (Low pH)  
514 (High pH)  
587 (Low pH)  
540 (High pH)  
Argon-Ion
Coriphosphine O
460  
575  
Argon-Ion
Coumarin-Phalloidin
387  
470  
Blue Diode
CPM (Coumarin-Maleimide)
385  
465  
Blue Diode
CTC Formazan
450  
630  
Argon-Ion
Cy2
489  
506  
Argon-Ion
Cy3
548  
562  
Green He-Ne
Cy3.1.8
554  
568  
Krypton-Argon
Cy3.5
581  
596  
Krypton-Argon
Cy5
650  
670  
Krypton-Argon
Cy5.1.8
649  
666  
Krypton-Argon
Cy5.5
675  
695  
Red Diode
Cy7
710  
805  
Near-Infrared
DANS
340  
525  
Helium-Cadmium
DANSA
340-380      
430  
Helium-Cadmium
Dansyl Cadaverine
335  
518  
Helium-Cadmium
Dansyl Chloride
335  
500  
Helium-Cadmium
Dansyl Hydrazine
336  
531  
Helium-Cadmium
DAO
(Diaminophenyloxydiazole)
280  
460  
Helium-Cadmium
DAPI
350  
470  
Helium-Cadmium
Dapoxyl
373  
574  
Argon-Ion
Di-4-ANEPPS
496  
705  
Argon-Ion
Di-8-ANEPPS
488  
605  
Argon-Ion
DiA
456  
590  
Argon-Ion
DiD (DilC18(5))
644  
665  
Krypton-Argon
DIDS
341  
414  
Helium-Cadmium
DiI-C18(3)
549  
565  
Green He-Ne
Dimethylamino-5-
Sulphonic Acid
310-370      
520  
Helium-Cadmium
DiO-C18(3)
484  
501  
Argon-Ion
Diphenyl Brilliant Flavine 7GFF
430  
520  
Blue Diode
DM-NERF
497 (Low pH)  
510 (High pH)  
527 (Low pH)  
536 (High pH)  
Argon-Ion
DPH (Diphenylhexatriene)
351  
430  
Helium-Cadmium
Dopamine
340  
490-520      
Helium-Cadmium
DsRFP (Ds Red Fluorescent Protein)
558  
583  
Green He-Ne
ELF-97 Alcohol
345  
530  
Helium-Cadmium
EBFP (Enhanced Blue FP)
380  
440  
Blue Diode
ECFP (Enhanced Cyan FP)
433, 453      
475, 501      
Blue Diode
EGFP (Enhanced GFP)
488  
507  
Argon-Ion
ERTracker Blue/White DPX
374  
575  
Argon-Ion
EYFP (Enhanced Yellow FP)
513  
527  
Argon-Ion
Eosin
525  
545  
Argon-Ion
Erythrosin Isothiocyanate
530  
558  
Argon-Ion
Ethidium Bromide
510  
595  
Argon-Ion
Ethidium Homodimer
528  
617  
Argon-Ion
Euchrysin
430  
540  
Blue Diode
Evans Blue
550  
610  
Green He-Ne
FAM (5-Carboxyfluorescein)
492  
518  
Argon-Ion
Fast Blue
360  
410  
Helium-Cadmium
FDA (Fluorescein Diacetate)
495  
520  
Blue Diode
Flazo Orange
375-530      
612  
Argon-Ion
Fluorescamine
390  
460  
Blue Diode
Fluorescein
494  
518  
Argon-Ion
Fluorescein Isothiocyanate (FITC)
490  
525  
Argon-Ion
Fluo-3
485  
503  
Argon-Ion
Fluo-4
494  
516  
Argon-Ion
FluoroEmerald
494  
520  
Argon-Ion
FluoroGold (Hydroxystilbamidine)
323 (Low pH)  
368 (High pH)  
408 (Low pH)  
565 (High pH)  
Helium-Cadmium
FluoroJade
425  
510  
Blue Diode
FluoroRuby
555  
582  
Krypton-Argon
FluorX
494  
520  
Argon-Ion
FM1-43
479  
598  
Argon-Ion
FM4-64
515  
640  
Argon-Ion
Formaldehyde Induced Fluorescence
405  
435  
Blue Diode
Fura-2
363 (Low [Ca])  
335 (High [Ca])  
512 (Low [Ca])  
505 (High [Ca])  
Helium-Cadmium
Fura Red
472 (Low [Ca])  
436 (High [Ca])  
657 (Low [Ca])  
637 (High [Ca])  
Argon-Ion
Genacryl Brilliant Red B
520  
590  
Argon-Ion
Genacryl Brilliant Yellow 10GF
430  
485  
Blue Diode
Genacryl Pink 3G
470  
583  
Argon-Ion
Genacryl Yellow 5GF
430  
475  
Blue Diode
GFP
(Green Fluorescent Protein)
395, 475      
510  
Blue Diode
Gloxalic Acid
405  
460  
Blue Diode
Granular Blue
355  
425  
Helium-Cadmium
Haematoporphyrin
530-560      
580  
Green He-Ne
Hoechst 33258, 33342
(Bound to DNA)
352  
461  
Helium-Cadmium
3-Hydroxypyrene-5,8,10-TriSulfonic Acid
403  
513  
Blue Diode
7-Hydroxy-4-methylcoumarin
360  
455  
Helium-Cadmium
5-Hydroxy-Tryptamine (5-HT)
380-415      
520-530      
Blue Diode
1,5-IAEDANS
336  
482  
Helium-Cadmium
Indo-1
350  
405-482      
Helium-Cadmium
Indodicarbocyanine
644  
665  
Krypton-Argon
Intrawhite Cf (Liquid)
360  
430  
Helium-Cadmium
JC-1
514  
529  
Argon-Ion
6-JOE
525  
555  
Argon-Ion
JOJO-1, JO-PRO-1
529  
545  
Argon-Ion
Leucophor PAF
370  
430  
Argon-Ion
Leucophor SF
380  
465  
Argon-Ion
Leucophor WS
395  
465  
Blue Diode
Lissamine Rhodamine B200 (RD200)
575  
595  
Krypton-Argon
LOLO-1, LO-PRO-1
565  
579  
Krypton-Argon
Lucifer Yellow CH
425  
528  
Blue Diode
Lucifer Yellow VS
430  
535  
Blue Diode
LysoSensor Blue DND-192, DND-167
374  
425  
Argon-Ion
LysoSensor Green DND-153, DND-189
442  
505  
Argon-Ion
LysoSensor Yellow/Blue
384 (Low pH)  
329 (High pH)  
540 (Low pH)  
440 (High pH)  
Helium-Cadmium
LysoTracker Blue
373  
422  
Argon-Ion
LysoTracker Blue/White
466  
536  
Argon-Ion
LysoTracker Green
504  
511  
Argon-Ion
LysoTracker Red
577  
590  
Krypton-Argon
LysoTracker Yellow HCK-123
465  
535  
Green He-Ne
Magdala Red
524  
600  
Argon-Ion
Magnesium Green
506  
531  
Argon-Ion
Magnesium Orange
550  
575  
Green He-Ne
Marina Blue
362  
459  
Argon-Ion
Maxilon Brilliant Flavin 10 GFF
450  
495  
Argon-Ion
Maxilon Brilliant Flavin 8 GFF
460  
495  
Argon-Ion
Merocyanine 540
500  
572  
Argon-Ion
4-Methylumbelliferone
360  
449  
Helium-Cadmium
Mithramycin
450  
570  
Argon-Ion
MitoFluor Green
490  
516  
Argon-Ion
MitoFluor Red 589
588  
622  
Yellow He-Ne
MitoFluor Red 594
598  
630  
Orange He-Ne
MitoFluor Far Red 680
680  
700  
Red Diode
MitoTracker Green FM
490  
516  
Argon-Ion
MitoTracker Orange CMTMRos
554  
576  
Krypton-Argon
MitoTracker Red CMXRos
579  
599  
Krypton-Argon
MitoTracker Red 580
581  
644  
Krypton-Argon
MitoTracker Deep Red 633
644  
665  
Krypton-Argon
MPS (Methyl Green Pyronine Stilbene)
364  
395  
Argon-Ion
Monochlorobimane
380  
461  
Blue Diode
Nano Orange
485  
590  
Argon-Ion
NBD
450  
530  
Argon-Ion
Nile Red
515-530      
525-605      
Argon-Ion
Nitrobenzoxadidole
460-470      
510-650      
Argon-Ion
Noradrenaline
340  
490-520      
Helium-Cadmium
Nuclear Fast Red
289-530      
580  
Argon-Ion
Nuclear Yellow
365  
495  
Argon-Ion
Nylosan Brilliant Flavin E8G
460  
510  
Argon-Ion
Olivomycin
430  
545  
Blue Diode
OPT (o-Phthalaldehyde)
340  
455  
Helium-Cadmium
Oregon Green 488
496  
524  
Argon-Ion
Oregon Green 500
503  
522  
Argon-Ion
Oregon Green 514
511  
530  
Argon-Ion
Pacific Blue
410  
455  
Blue Diode
Pararosaniline (Feulgen)
570  
625  
Krypton-Argon
PE-B (Phycoerythrin B)
545  
576  
Green He-Ne
PE-R (Phycoerythrin R)
490, 565      
578  
Argon-Ion
Phorwite AR (Solution)
360  
430  
Helium-Cadmium
Phorwite BKL
370  
430  
Argon-Ion
Phorwite Rev
380  
430  
Argon-Ion
Phorwite RPA
375  
430  
Argon-Ion
Phosphine 3R
465  
565  
Argon-Ion
Phosphine R
480-565      
578  
Argon-Ion
Phycocyanin C
620  
648  
Orange He-Ne
Phycocyanin R
618  
642  
Orange He-Ne
PKH26
551  
567  
Green He-Ne
Pontochrome Blue Black
535-553      
605  
Green He-Ne
POPO-1, PO-PRO-1
434  
456  
Blue Diode
POPO-3, PO-PRO-3
534  
572  
Green He-Ne
Primuline O
410  
550  
Blue Diode
Procion Yellow
470  
600  
Argon-Ion
Propidium Iodide
536  
617  
Green He-Ne
PyMPO
415  
570  
Blue Diode
Pyrene
345  
378  
Helium-Cadmium
Pyronin Y
540  
570  
Green He-Ne
Pyronine
410  
540  
Blue Diode
Pyronine B
540-590      
560-650      
Krypton-Argon
Pyrozal Brilliant Flavin 7GF
365  
495  
Argon-Ion
Qdot 525
350-450      
525  
Blue Diode
Qdot 525
350-525      
565  
Blue Diode
Qdot 585
350-550      
585  
Blue Diode
Qdot 605
350-575      
605  
Blue Diode
Qdot 655
350-600      
655  
Blue Diode
Quinacrine Mustard
423  
503  
Blue Diode
Resorufin
570  
585  
Krypton-Argon
RH 414
532  
716  
Argon-Ion
Rhod-2
550  
571  
Green He-Ne
Rhodamine 110
496  
520  
Argon-Ion
Rhodamine 123
511  
534  
Argon-Ion
Rhodamine 5 GLD
470  
565  
Argon-Ion
Rhodamine 6G
526  
555  
Argon-Ion
Rhodamine B
540  
625  
Green He-Ne
Rhodamine B 200
523-557      
595  
Green He-Ne
Rhodamine B Extra
550  
605  
Green He-Ne
Rhodamine BB
540  
580  
Green He-Ne
Rhodamine BG
540  
572  
Green He-Ne
Rhodamine Green
502  
527  
Argon-Ion
Rhodamine Red
570  
590  
Krypton-Argon
Rhodamine WT
530  
555  
Argon-Ion
Rhodol Green
499  
525  
Argon-Ion
RITC (Rhodamine B Isothiocyanate)
570  
595  
Krypton-Argon
Rose Bengal
540  
550-600      
Green He-Ne
ROX (Carboxy-X-Rhodamine)
574  
602  
Krypton-Argon
SBFI
334  
525  
Helium-Cadmium
Serotonin
365  
520-540      
Argon-Ion
Sevron Brilliant Red 2B
520  
595  
Argon-Ion
Sevron Brilliant Red 4G
500  
583  
Argon-Ion
Sevron Brilliant Red B
530  
590  
Argon-Ion
Sevron Orange
440  
530  
Blue Diode
Sevron Yellow L
430  
490  
Blue Diode
SITS (Stilbeneisothiosulphonic Acid)
337  
436  
Helium-Cadmium
Sodium Green
507  
535  
Argon-Ion
Spectrum Aqua
433  
480  
Blue Diode
Spectrum Green
497  
524  
Argon-Ion
Spectrum Orange
559  
588  
Krypton-Argon
Spectrum Red
604  
624  
Orange He-Ne
Stilbene
335  
440  
Helium-Cadmium
Sulforhodamine 101
586  
605  
Yellow He-Ne
Sulforhodamine B
565  
586  
Krypton-Argon
Sulforhodamine G
529  
548  
Argon-Ion
SYTOX Blue
444  
480  
Blue Diode
SYTOX Green
504  
523  
Argon-Ion
SYTOX Orange
547  
570  
Green He-Ne
TAMRA (Carboxytetramethylrhodamine)
542  
568  
Green He-Ne
Tetracycline
390  
560  
Blue Diode
Texas Red
596  
615  
Yellow He-Ne
Thiazine Red R
510  
580  
Argon-Ion
Thiazole Orange
453  
480  
Argon-Ion
Thioflavin S
430  
550  
Blue Diode
Thioflavin TCN
350  
460  
Helium-Cadmium
Thiolyte
370-385      
477-484      
Argon-Ion
Tinopol CBS
390  
430  
Blue Diode
TOTO 1, TO-RRO-1
514  
533  
Argon-Ion
TOTO 3, TO-PRO-3
642  
661  
Krypton-Argon
TRITC
557  
576  
Krypton-Argon
True Blue
365  
420-430      
Argon-Ion
Ultralite
656  
678  
Krypton-Argon
Uranine B
420  
520  
Blue Diode
Uvitex SFC
365  
435  
Argon-Ion
X-Rhodamine
580  
605  
Krypton-Argon
Xylenol Orange
546  
580  
Green He-Ne
XRITC (X-Rhodamine Isothiocyanate)
582  
601  
Krypton-Argon
YOYO-1, YO-PRO-1
491  
509  
Argon-Ion
YOYO-3, YO-PRO-3
612  
631  
Orange He-Ne

虽然在本表数据已被检查的准确性,它是强烈呼吁,为一个特定的荧光性质由第一咨询染料制造商确认安全的吸收和发射光谱分布全规范展开实验之前。 此外,建议的激光器是打算作为一个参考,并在许多情况下,替代照明源可以产生更好的结果。